Title: Fully integrated microfluidic device transfigured from conventional 96- well ELISA kit

Abstract

ELISA using 96-well microplate has been widely used as a standard clinical diagnostics method, but it requires expensive materials with prolonged incubation time. In this work, a fully integrated active microfluidic device employing a conventional 96-well kit was implemented to improve the performance of traditional enzyme linked immunosorbent assay (ELISA). The programmable and disposable on-chip pump and valve of the device precisely regulated a reaction time using optimized reagent volume to execute ELISA with consumption of reduced sample and reagents shortening the assay time, which are crucial for conventional ELISA using 96-well microplate. This unconventional device was successfully applied for the detection of cardiac troponin I (cTnI) of 4.88 pg/mL using minimum sample volume of 30 µL with shorter assay time of 15 min for each ELISA step.