The aim is to evaluate the oxidant and antimicrobial effects of Fe3O4, TiO2 nanoparticles and thalicarpine by luminescent and standard microbiological assays. Their effect is studied on the kinetics of free-radical oxidation reactions (at pH 7.4 and pH 8.5) in model systems, using activated chemiluminescence: * chemical, with Fenton`s reagent (H2O2-FeSO4) - for the generation of hydroxyl radicals (.OH) * chemical, with oxidant hydrogen peroxide (H2O2) * chemical (NAD.H-PhMS), for the generation of superoxide radicals (O2.-). Fe3O4 nanoparticles exhibit highly pronounced antioxidant properties; TiO2 nanoparticles exhibit mild to moderate prooxidant properties at neutral and alkaline conditions. Those properties are tested by the chemiluminescent method for the first time. Thalicarpine and its combination with TiO2 nanoparticles exhibit pronounced antioxidant activities at pH 8.5 which are lost and transformed into well-presented prooxidant effects at pH 7.4. That is a result-supported proof on the observed typical properties of thalicarpine and TiO2, namely antibacterial, organic-preserving and anti-pathogenic activities. The antimicrobial effect is tested on Gram-positive and Gram-negative bacteria: two strains of Escherichia coli, Bacillus cereus 1095 and Staphylococcus aureus. All bacteria are destroyed after the application of TiO2, but not Fe3O4 nanoparticles, showing their antibacterial effect. Thalicarpine, in combination with TiO2, showed even synergetic antibacterial effect.
Elitsa Pavlova graduated as a Master in Biology at Sofia University "St. Kliment Ohridski", Bulgaria in 2001 specializing in Cellular biology and biology of development. In 2002, she completed a second M.Sc. in Biology and chemistry, same university. Her PhD thesis is in Human and animal physiology (2007), focusing on the assessment of biomarkers of oxidative stress in patients with different diseases. She has been working in the field of Biophysics with experimental and clinical laboratory data, statistical analysis and the evaluation of pathological effects ex vivo, in vitro and in vivo. She is a professional in the evaluation of nutritional supplements and antioxidants, medicinal substances and nanomaterials, their combinations and side effects. She currently teaches in the university and researches on national and international projects.She is well known for her achievements in the fields of Biochemistry and Toxicology, Biophysics and Medical Physics.
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