Title: Green synthesis and characterize of silver nanoparticles by using leaves extract of Polyalthia Sclerophylla and their cytotoxicity studies

Abstract

This study was conducted to synthesize and characterize Silver Nanoparticles (AgNPs) using a rapid green synthesis approach. The crude extract of leaves of Polyalthia sclerophylla (CEPS) were used as a reducer and silver nitrate (AgNO3) as an initiator. Two samples were prepared and named as AgNPs-a and AgNPs-b, respectively. The prepared samples were carried out to characteristic their chemical, physical and biological properties. The first technique that used to confirm the formation of AgNPs were ultraviolet–visible spectroscopy (Uv-vis) and Energy Dispersive X-Ray Analysis (EDX). Scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) were used to identify the morphology and size of the particles. Mg-63 human cells were used to study the cytotoxicity of the AgNPs using the Alamar blue assay. The results have been shown that, the wavelength of AgNPs-a was 436 nm, while the AgNPs-b showed at 441 nm. Prepared samples were shown spherical in their shapes according to SEM and STEM images. The size of the particles was not same, whereas, the diameter size range of AgNPs-a were from 48nm to 68 nm, while the AgNPs-b showed range from 59 nm to 77 nm. Prepared samples showed availability for the Mg-63 cells more than 89% for all concentrations after 24h. Present study has been demonstrated non-toxic prepared samples with nano-sizes which can be used safely in the medical and biological fields.